FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColTGA5_pool19011C_B_e-1i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColTGA5_pool19011C_B_e-1i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65494
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1440.2198674687757657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTTC252.8111517E-670.000011
CCTACTG252.8111517E-670.000017
TGAGTCC500.070.000012
GCCATCC500.070.000012
GTTGTCC252.8111517E-670.000012
AGGATCC455.456968E-1270.000012
CTAGAAT150.00221551670.08
GCAAGTC207.872973E-570.01
CCTAGTC150.00221551670.07
TCCTGTG301.0092663E-770.06
TCCTGTC600.070.06
CCTAGCC207.872973E-570.07
GGGTCCT550.070.03
GTCCTTT1200.070.05
CCTACGA150.00221551670.07
CCTACCT150.00221551670.07
CCTACCA353.6416168E-970.07
AGGGTCC207.872973E-570.02
TCCTAGC1050.070.06
TGCATCC207.872973E-570.02
Produced by FastQC (version 0.11.5)