FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColTGA5_pool19011C_B_e-1h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColTGA5_pool19011C_B_e-1h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261631
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3910.14944712209180105No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTCC1400.070.000762
GGTCCTA6000.068.834084
GTCCTAC4250.068.353685
AGTCCTA9650.067.824584
GTCCTAT10500.067.334075
AGGTCCT6800.066.91253
AAGTCCT6750.066.889623
GTTCCTA7100.066.5500264
GTCCTAA7850.065.987985
GATTCCT6550.065.725913
GGGTCCT2400.065.625723
ACGATCC2400.065.625722
TCCTATA12750.065.608566
TCCTAGA9300.065.484596
TCCTATG8350.065.389946
TAGTCCT6800.065.368363
AGTCCTG3000.065.3340454
CTGTCCT3000.065.3340453
GTCCTAG5250.065.3340455
TGTCCTA8200.065.305594
Produced by FastQC (version 0.11.5)