FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColTGA5_pool19011C_B_e-1g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColTGA5_pool19011C_B_e-1g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100930
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2750.27246606559001285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGCTC150.002215085670.03123554
CGTTCCT1500.069.996543
ACGATCC1300.069.996542
CTAGACT150.002219447469.996538
CTATCGT252.8230734E-669.996538
GATCCTA4350.069.996534
CCTGTAC150.002219447469.996537
CCTAAGG600.069.996537
CGCTCCT900.069.996533
AGCGCTC150.002219447469.996531
AGCGATC207.895611E-569.996531
GCTAGTC150.002219447469.996531
GCGATCC301.0151962E-769.996532
CTATTCG207.895611E-569.996538
CCTGGGA150.002219447469.996537
CCTGGCC150.002219447469.996537
CCTGGCA252.8230734E-669.996537
CGATCCT2750.069.996533
GCGTATC150.002219447469.996531
TCGGTCC301.0151962E-769.996532
Produced by FastQC (version 0.11.5)