FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColTGA5_pool19011C_B_e-1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColTGA5_pool19011C_B_e-1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534038
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8830.16534403918822257No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG13400.067.3759845
CGGTCCT5200.067.295633
GTCCTAT18100.067.087425
AGTCCTA17850.066.850764
TGTCCTA16800.066.654724
GATCCTA23950.066.042444
ACGGTCC2650.066.02592
GGTCCTA12900.065.918414
CCTAGCG850.065.870547
GTCCTAC8350.065.7965855
TCCTATT26100.065.562966
CGTCCTA7900.065.557864
GGTCCTT7550.065.352524
TGGTCCT9800.065.345433
ATCCTAT30250.065.128825
TCCTATA27900.065.0958566
GGTCCTC7150.065.093224
GTCCTAA15600.065.052445
GGGTCCT4200.064.988353
TAGTCCT12450.064.9281163
Produced by FastQC (version 0.11.5)