FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColTGA5_pool19011C_B_e-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColTGA5_pool19011C_B_e-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370199
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10960.29605698556722193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCC455.456968E-1269.9949952
GGTCCTA8250.067.873954
GTCCTAT14600.067.35825
TGGTCCT6600.066.283153
AGTCCTA13050.066.240484
GATCCTA17500.066.1952744
TCCTATG11750.066.122946
GTCCTAG8550.065.9017265
AGTCCTG4000.065.6203164
CGGTCCT3800.065.390073
GTCCTAC4500.065.328675
ATTCCTA14300.065.100244
GGTCCTT4850.064.943824
ATCCTAT21400.064.925275
TCCTATA19900.064.894866
GAGTCCT9500.064.837483
TGTCCTA12800.064.800064
TAGTCCT9950.064.7189943
TTCCTAT15600.064.610775
GTCCTTT7100.064.572855
Produced by FastQC (version 0.11.5)