FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColTGA5_pool19011C_B_e-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColTGA5_pool19011C_B_e-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118014
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42240.19943210951391255No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCTA53500.067.900494
GTCCTAT88400.067.620015
CGGTCCT20700.067.118043
AGTCCTA79600.066.7842564
GTCCTAG42150.066.591135
AGGTCCT53350.066.384273
GTCCTAA69100.066.298125
GGGTCCT17600.065.8164443
TGTCCTA68200.065.785934
CGTCCTA31250.065.626164
TAGTCCT58600.065.51313
GATCCTA101100.065.3205954
ATCCTAT123650.065.1554645
GAGTCCT57600.065.131553
TCCTATA109950.064.998026
GGTCCTC29550.064.901154
TCCTATG70600.064.7903756
CGATCCT53700.064.778553
GTCCTAC32850.064.561985
ACGTCCT31900.064.5068363
Produced by FastQC (version 0.11.5)