FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColTGA5_pool19011C_B_e-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColTGA5_pool19011C_B_e-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914534
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28080.3070416190103375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCT9750.067.478693
GTCCTAT34700.066.868455
AGTCCTA33050.066.500574
GGTCCTA20500.065.897764
GATCCTA41800.065.1389164
AGGTCCT19450.065.1331943
GTCCTAA29000.065.04715
TGTCCTA28850.065.021374
GAGTCCT23000.064.9700853
GTCCTAC12950.064.860255
GTCCTAG18500.064.69815
ATCCTAT50800.064.6902855
TAGTCCT25800.064.565523
TCCTATA43700.064.552726
GGTCCTC12450.064.372934
GTTCCTA24250.064.3665544
TCCTAAC19700.064.313666
TCCTATG30400.064.242376
CGATCCT23400.064.158593
TCCTAGC12750.063.9597366
Produced by FastQC (version 0.11.5)