FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColTGA5_pool19011C_B_e-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColTGA5_pool19011C_B_e-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10493616
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG192870.18379746314330542No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT416450.067.047485
GGTCCTA243850.066.881534
CGGTCCT97550.066.407963
AGGTCCT234150.066.288853
AGTCCTA387400.066.201664
TGTCCTA328300.065.859744
GAGTCCT256000.065.798643
GTCCTAA337650.065.654225
GTCCTAC151150.065.505255
GATCCTA491850.065.434884
TAGTCCT281700.065.4112553
GTCCTAG204450.065.409565
CGTCCTA150950.064.848874
TCCTATA539400.064.8325046
ATCCTAT622850.064.737815
GGTCCTC136150.064.726354
CGATCCT271500.064.568713
GTTCCTA266450.064.47934
GGGTCCT81800.064.134143
TCCTAGA377850.064.069256
Produced by FastQC (version 0.11.5)