FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColTGA5_pool19011B_B_e-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColTGA5_pool19011B_B_e-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162230
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACTA150.002216481470.03950559
GGGTCCT353.6889105E-969.99633
GCGCTAA150.00222191569.99635
GGTCCTC252.8308805E-669.996294
CGCTATA252.8308805E-669.996296
GTCCTCT850.061.761445
GAGCGCT252.3855171E-455.9970363
GCGATCC252.3855171E-455.9970362
ACCTCCT452.685374E-854.4415633
AGTGCTA200.006925846552.5296359
GTACCTG200.00693430752.5134268
GTGTTCA200.006942775652.4972235
CGCGCTC200.006942775652.4972234
CACGCCT200.006942775652.49722353
CCGCTAT200.006942775652.4972235
AGCGCTG200.006942775652.4972234
ACGTCCT200.006942775652.4972233
GCGCTAT200.006942775652.4972235
CCTGAAT407.446888E-752.4972237
TGGTCCT407.446888E-752.4972233
Produced by FastQC (version 0.11.5)