FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColTGA5_pool19011B_B_e-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColTGA5_pool19011B_B_e-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4312941
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT30550.054.533935
AGTCCTA31750.050.4879574
GGTCCTA18000.048.9997754
AGGTCCT19000.047.1565783
GAGTCCT21950.046.3996243
GATCCTA40250.046.17374
GGGTCCT6000.045.4987343
TGTCCTA30450.045.1722034
GTCCTAG15700.045.032165
GGCGCTA3300.044.5452464
GTCCTAA32500.044.2618455
CGATCCT20000.043.923783
GTCCTCT23200.043.9011655
TAGTCCT23850.043.730443
CGGTCCT7850.043.693053
GTCCTGT13500.043.555865
TCCTATA49600.043.397486
TCCTAGA33000.043.0609056
CATCCTA32300.043.018384
ATGTCCT28400.043.0093653
Produced by FastQC (version 0.11.5)