FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_input_e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_input_e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14742
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTCA150.00218134870.000015
TCCTCAC150.00218134870.000016
ACGCTAT150.00218134870.000015
CCTATAC150.00218134870.000017
CTATAAC150.00218134870.000018
AGCTATA150.00218134870.000016
GGGTAAT150.00218134870.000013
CCTCGCT150.00218134870.000013
CCGCTCA150.00218134870.000015
CGCTAAT150.00218134870.000016
CAATAAT150.00218134870.000013
TATCCTT150.00218134870.000014
CGGCGCT150.00218134870.000013
GAATCCT150.00218134870.000013
GCGTCGA150.00218134870.0000128
CCTTAAT150.00218134870.000013
AGCGCTA150.00218134870.000014
AGTCCTA309.557152E-870.000014
GTAATCT150.00218134870.000015
CTCGCTA150.00218134870.000014
Produced by FastQC (version 0.11.5)