FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_input_e-1i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_input_e-1i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378233
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4560.120560606821721No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG2150.065.112716
GACCGCT1400.064.996443
CGCTAAC1700.063.8200346
TCGCTAT3100.063.2223435
CACCGCT2050.063.1672743
GATCGCT1500.062.9965483
CGCTAGT3000.062.9965486
GGCGCTA3450.062.8951074
GCGCTAG3250.062.4581155
CGCTATT4800.061.9757736
CCGCTAT3850.061.8147965
CGCTCAT2900.061.5483556
GCGCTAT4750.061.1545455
GGTAATC5100.061.0750854
GCCCGCT1150.060.866233
CGCTATC2650.060.751396
GTAATCT5950.060.5849155
GCGACGC1050.059.996712
CCCGCTA2050.059.7528274
GAGTAAT5000.059.4967423
Produced by FastQC (version 0.11.5)