FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_input_e-1h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_input_e-1h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213822
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG150.002223540369.991111
ACACGCT207.918863E-569.991113
ACGTTCC150.002223540369.991112
ACCGCGC207.918863E-569.991112
CGCGCAC207.918863E-569.991114
CCGCTGG150.002223540369.991115
CGCTGAA301.02119884E-769.991116
CCGCTCC207.918863E-569.991115
TGCCTAA150.002223540369.991112
TCCCGCT207.918863E-569.991113
CGCACGT150.002223540369.991116
AACCGCT850.065.8739853
CGCTAAC850.065.8739856
CGGTAAT2100.064.9917453
ACGCTAT1350.064.806585
TTCGCTA1350.064.806584
CCCGCTA650.064.607184
ATCCAGG600.064.1585165
CGCTATA2250.063.769686
GTACGCT550.063.628283
Produced by FastQC (version 0.11.5)