FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_input_e-1g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_input_e-1g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47719
Sequences flagged as poor quality0
Sequence length76
%GC40

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTGT207.851091E-570.000015
GTCGCTA301.00337274E-770.000014
CGACGCT150.00221181870.000013
GCGGTAA150.00221181870.000012
TCCTGTC150.00221181870.000016
TCTTCGC207.851091E-570.000012
GAGTAAT401.3096724E-1070.000013
GGTCCTA301.00337274E-770.000014
GTCTCGC150.00221181870.000012
CCCAGCG150.00221181870.000011
TTCGTAA150.00221181870.000012
CCGCTTT150.00221181870.000015
CGCTGAC207.851091E-570.000016
CCTAAAG150.00221181870.000017
AGCGGCT207.851091E-570.000011
TACGCTA150.00221181870.000014
GGGACGC150.00221181870.000012
CGCTCAG207.851091E-570.000016
GTTCGCT207.851091E-570.000013
CGCTAGA301.00337274E-770.000016
Produced by FastQC (version 0.11.5)