FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_input_e-1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_input_e-1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36530
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGC207.8263845E-570.02
TAATAGG150.002207638570.06
GTCGCTC252.7862352E-670.04
CGGCGCT150.002207638570.03
AGCCGCT150.002207638570.03
GCGACGC150.002207638570.02
CTTCCGA207.8263845E-570.010
CAACGCT150.002207638570.03
CGACGCT150.002207638570.03
GTCCTAT353.5815901E-970.05
GTCCTAC150.002207638570.05
GACCGCT207.8263845E-570.03
TGGCGCT207.8263845E-570.03
CGCGCTT150.002207638570.04
CCAATAA150.002207638570.02
GCCGGGT150.002207638570.064
GCACGCT150.002207638570.03
CCAGCGC252.7862352E-670.02
CTCTTCC150.002207638570.08
CGCTGTT252.7862352E-670.06
Produced by FastQC (version 0.11.5)