FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_input_e-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_input_e-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2062130
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20760.10067260550983691No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCTA19950.062.631364
GGCGCTC15600.062.1472244
CGGTAAT26600.061.8418923
AGGCGCT12600.061.11093
CCGCTAT18800.061.0636185
CGCTATA24650.060.9125676
GCGCTAG16800.060.2081225
CGCTATT24300.059.917496
CGCTAGT13500.059.888686
GGGCGCT12200.059.6719253
CGGCGCT12150.059.341363
CCCGCTA12500.059.0797964
CCCCGCT8950.059.050083
CGCTAAC9800.058.928376
ACCGCTA15600.058.781854
GGTAATC29200.058.7326744
CTGCGCT8200.058.4754073
CCGCTAG12750.058.4703835
CGCTCAA15400.058.4088866
CGCTCAT16900.058.4021646
Produced by FastQC (version 0.11.5)