FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_input_e-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_input_e-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587568
Sequences flagged as poor quality0
Sequence length76
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAT12700.063.3843425
CGGTAAT18050.062.0484123
GGGCGCT7350.061.9033163
AGGCGCT9250.061.6742323
GGCGCTC10700.061.4938934
CGGCGCT8200.061.0351563
CGCTATT17150.061.0189826
GGCGCTA15300.060.3907434
CGCTCAA11200.060.3110926
AGGTCCT8950.060.2220543
GTCCGCT5150.059.804433
CCGCTCT12150.059.6282355
TCGCTAT14850.059.392555
CCCGCTA7250.059.3779224
CGCTATA18250.059.2588846
CGCTAGT8550.058.945996
TGCCGCT6650.058.9459883
CGCTCTT24650.058.6396036
CCCCGCT5850.058.6311073
TGGCGCT10850.058.385733
Produced by FastQC (version 0.11.5)