FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_input_e-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_input_e-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580801
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGCG150.002224250770.000781
GGCGCTC3950.065.570354
CCGGTAA2800.063.7507062
CCCGCTA3900.063.718664
TGGCGCT4050.063.087123
CGCTAGT4000.063.00076
GCCCGCT1500.063.00073
TGCGCTA2950.062.882054
CGGTAAT7350.062.8578383
CCGCTAT5450.062.294275
CTGCGCT2200.062.0461433
CGGCGCT3850.061.8188633
CACCGCT3600.061.250683
CGCGCTC2650.060.7553944
AGGCGCT3350.060.5976833
GGGCGCT2500.060.200663
GGCGCTA5850.059.8297234
CGCTATA6850.059.781686
GCGCTAG3750.059.7339945
GCGTAAT3650.059.4527133
Produced by FastQC (version 0.11.5)