FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_input_e-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_input_e-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743921
Sequences flagged as poor quality0
Sequence length76
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCTC3400.066.9096454
CCGCTAT6050.063.055855
GCCGCTA4900.062.8551524
GGGCGCT3550.062.1107063
CCCCGCT2400.061.2480623
CCCCCGC800.061.2439422
GCGGCCC401.067383E-861.2439422
AGCCGCT2750.061.0889743
GCGCTAG5100.061.07655
CCGCTAC1950.061.023715
AGGTCCT3900.061.023713
GGCGCTA6600.060.452634
AGGCGCT3650.060.4090463
CGCTACT3800.059.8665246
CGGTAAT7850.059.743333
CGCCGCT3050.059.6702423
TGCGCTC2350.059.5725824
CCCGCTA3650.059.4501724
TGCGCTA3200.059.0606274
CCGCTAG4100.058.9005745
Produced by FastQC (version 0.11.5)