FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_UB_e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_UB_e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7358
Sequences flagged as poor quality0
Sequence length76
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAA150.002137746770.000015
GGCGCTA150.002137746770.000014
CCGCTAA150.002137746770.000015
CGCTCCT150.002137746770.000016
AATTAAT150.002137746770.000013
CGCTATT150.002137746770.000016
CGCTATC150.002137746770.000016
CGCTAGC150.002137746770.000016
TGTTAAT150.002137746770.000013
TAATCTG150.002137746770.000016
TAATCTA150.002137746770.000016
TAATAGC150.002137746770.000016
AGTCCTG150.002137746770.000014
AGCCGCT150.002137746770.000013
GTAATCT150.002137746770.000015
GTAATAA150.002137746770.000015
GTTAATC150.002137746770.000014
GGTAATA150.002137746770.000014
AGATAAT150.002137746770.000013
GGCGCTC207.417047E-570.04
Produced by FastQC (version 0.11.5)