FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_UB_e-1i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_UB_e-1i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140651
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAA252.8280429E-670.02
CGTAATG750.070.04
ACGTAAT750.070.03
GGCGCTA2200.068.409094
TAATCGG1150.066.956536
TTAATAG1050.066.666675
GTAATAG2650.066.0377355
GCGCTAT2150.065.116285
GCGCTAA1400.065.05
AGGTAAT1950.064.615393
TCGTAAT1250.064.43
TAATAGG1200.064.1666646
GTAATCT3400.063.823535
GCCGCTA2250.063.777784
ATAATAT1600.063.4374965
GTAATAC1050.063.3333365
CGCTATA2100.063.3333366
CGCTAGA2100.063.3333366
TGCCGCT1050.063.3333363
CGTAATA1550.063.225814
Produced by FastQC (version 0.11.5)