FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_UB_e-1h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_UB_e-1h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48510
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCTC650.070.000016
CGCTATC650.070.000016
CTTAATG207.8524215E-570.04
CTTAATC353.614332E-970.04
ATCTCGT150.002212043470.08
TTAATGC252.8001523E-670.05
TTAATAC150.002212043470.05
GATCGCT207.8524215E-570.03
CACATGA150.002212043470.028
GCTATCA150.002212043470.07
GCTATAG150.002212043470.07
CGCTGTA252.8001523E-670.06
CCGCTTT150.002212043470.05
CCTGTAG150.002212043470.07
CCTGTAA252.8001523E-670.02
CGCTGGC150.002212043470.06
TCCTATG150.002212043470.06
CCGCTAC301.00373654E-770.05
GCCGCTA401.3096724E-1070.04
AGAGTCC301.00373654E-770.02
Produced by FastQC (version 0.11.5)