FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_UB_e-1g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_UB_e-1g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27243
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAT207.7905E-570.03
TAATCAC150.00220156170.06
CAGGTAA150.00220156170.02
CGGGTAA207.7905E-570.02
AAGTAAT207.7905E-570.03
TAATAGC252.7670867E-670.06
TAATAGA252.7670867E-670.06
GGTAATC500.070.04
GGTGTAA150.00220156170.02
CAACGCT150.00220156170.03
CGTGCGC353.5361154E-970.02
ACCGCTA150.00220156170.04
ATAATCG207.7905E-570.05
ATAATAA550.070.05
CGCATAA150.00220156170.02
GCTATGG150.00220156170.07
GCTATAC150.00220156170.07
TGGCGCT353.5361154E-970.03
CACGCTA207.7905E-570.04
CGACCGC150.00220156170.02
Produced by FastQC (version 0.11.5)