FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_UB_e-1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_UB_e-1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14419
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCCT150.002180434870.03
CGATAAT207.6661956E-570.03
TTCGCTA207.6661956E-570.04
TTGTAAT252.7010483E-670.03
GGTCCTA150.002180434870.04
CGCTCTG207.6661956E-570.06
TCGGTAA150.002180434870.02
CCGCTAT150.002180434870.05
CCGCTAA150.002180434870.05
GATAATA252.7010483E-670.04
TAATGTT353.3796823E-970.06
CGCTATT252.7010483E-670.06
CGCTAGC207.6661956E-570.06
CGCTAGA207.6661956E-570.06
CGCTACT150.002180434870.06
CGCTACC150.002180434870.06
CATTAAT150.002180434870.03
CGGCGCT150.002180434870.03
GTCCTAT207.6661956E-570.05
AATAGAA150.002180434870.07
Produced by FastQC (version 0.11.5)