FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_UB_e-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_UB_e-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097806
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAG13500.064.552325
GGCGCTA17200.064.502594
CGCTAGA15250.063.5734836
CGCTAGT12400.062.378756
CGCTATT17200.062.063676
CCGCTAG12550.061.909255
GGGCGCT11100.061.7987063
GTAATCA17100.061.60515
AGGTAAT17800.061.5418623
GTAATAG17350.061.5242965
GCGCTAT17850.061.1734055
CGCTAGC9300.060.967466
TGCGCTA10850.060.964694
AGTAATC17450.060.570034
GTAATAC11450.060.520995
GTGTAAT12800.060.1532363
GTAATAT25100.060.0965925
GGTAATA26200.059.8443264
CCGCTAT15850.059.8392755
TAATAGA18900.059.814546
Produced by FastQC (version 0.11.5)