FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_UB_e-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_UB_e-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556073
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGA8200.066.151996
GCGCTAG6600.065.220825
CCGCTAG6900.064.921115
GTAATAG8050.063.906725
CGCTAGT5850.063.412536
GGCGCTA8550.062.6253854
AGGTAAT9200.062.3851283
CGCTAGG3550.062.1065336
GGTAATG7950.061.6291284
GGTAATA12650.061.416854
TCGCTAG5250.061.3272675
CAGCGCT5650.060.701963
GGATAAT7950.060.3085023
CGCTAAA6850.060.2860076
GATAATC7600.059.8624954
CCGCTAT8250.059.8122635
TAATAGT6800.059.6999786
AGCGCTA7100.059.6419874
GGCGCTC6400.059.603484
GGTAATC11650.059.4790954
Produced by FastQC (version 0.11.5)