FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_UB_e-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_UB_e-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454285
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATT6650.065.263166
CGCTAGG2600.064.615396
GTAATAC5400.062.870375
GCGCTCT7000.062.55
AGGTAAT8500.062.1764683
GCGCTAG4650.061.720435
CGCTAGT4350.061.149426
GGCGCTC5900.061.1016964
CCGCTAG4300.061.0465135
CGCTAGA6150.060.894316
TCGCTAG4600.060.8695645
TGCGCTA4100.060.6097564
GGGCGCT4450.060.56183
CGCTAAC3700.060.5405436
GCGTAAT3600.060.2777823
CGCTAAA6100.060.2459036
GTAATAG7850.060.1910825
CGCTATA6350.060.0787436
GGCGCTA6800.059.7058834
GCGCTAT7200.059.3055575
Produced by FastQC (version 0.11.5)