FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_UB_e-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_UB_e-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405537
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAT6450.066.201555
AGGTAAT6650.064.2105263
GGTAATG5300.063.396234
GCGCTAG3650.063.287675
GTAATAG5600.063.1255
TGCGCTA3350.062.686574
CAGCGCT4450.062.134833
CGCTATT6950.061.942446
GTAATGT5200.061.9230775
TAATAGC3650.061.369866
CGCTAGA5250.061.3333326
GGCGCTC4700.061.063834
TCGCTAG3850.060.9090965
GGCGCTA4450.060.56184
CGCTAAC3250.060.307696
AGCGCTA4950.060.1010064
ACGTAAT2950.059.3220333
CGCTATG3900.059.230776
GGTAATA8000.059.0625044
CCGCTAT5700.058.947375
Produced by FastQC (version 0.11.5)