FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_B_e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_B_e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7842
Sequences flagged as poor quality0
Sequence length76
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCAGA150.002142751870.06
TGTCCTA150.002142751870.04
ACGTAAT150.002142751870.03
ACTCCCA150.002142751870.04
TTATAGC150.002142751870.044
ATAATCC150.002142751870.05
ATAATAA150.002142751870.05
GGATAAT150.002142751870.03
TATGGAT150.002142751870.09
TAAATTA352.608649E-760.06
CCCAGAT252.2463687E-455.9999967
TAATATG252.2463687E-455.9999966
TAGCGCT200.006697261752.53
CTCCCAG200.006697261752.55
TAATTAC200.006697261752.56
TAATAAT200.006697261752.56
GTAATTA200.006697261752.55
GGTAATA200.006697261752.54
CATAATC200.006697261752.54
GATAATA305.5257324E-446.6666644
Produced by FastQC (version 0.11.5)