FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_B_e-1i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_B_e-1i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50491
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGCCGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG2070.40997405478204035Illumina Single End PCR Primer 1 (100% over 41bp)
AGCGGCGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG790.15646352815353232Illumina Single End PCR Primer 1 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT401.3096724E-1069.963333
TTTAGCG252.8105242E-669.963331
TAATACG207.875898E-569.963336
CCTTAAT207.875898E-569.963333
ACTGCGC207.875898E-569.963332
GCGACGC401.3096724E-1069.963332
CCCTCGC207.875898E-569.963332
AATCACT207.875898E-569.963337
GAGTCCC252.8105242E-669.963333
ACCGCTC500.069.963334
AAGCGCT401.3096724E-1069.963333
TCCTATC500.069.963336
CCGCTAC401.3096724E-1069.963335
TCCTAAC207.875898E-569.963336
CGCTCTA252.8105242E-669.963336
TGCGCTA401.3096724E-1069.963334
CCTCCTG207.875898E-569.963334
TCCAGGA401.3096724E-1069.963336
CAGATAA207.875898E-569.963332
CGCTAGC252.8105242E-669.963336
Produced by FastQC (version 0.11.5)