FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_B_e-1h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_B_e-1h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18395
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGCCGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG650.35335689045936397Illumina Single End PCR Primer 1 (100% over 41bp)
AGCGACGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG310.1685240554498505Illumina Single End PCR Primer 1 (100% over 41bp)
AGCGCCGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCG230.12503397662408264Illumina Single End PCR Primer 1 (97% over 41bp)
AGCGGCGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG210.11416145691764065Illumina Single End PCR Primer 1 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGGT252.731109E-670.0000149
GCGGCGC252.731109E-670.000012
CCGTAAT252.731109E-670.000013
AGCGGCG252.731109E-670.000011
TGTAGAA252.731109E-670.0000140
GGTAGCC252.731109E-670.0000153
TGCTAAT252.731109E-670.000013
GTGGTAG252.731109E-670.0000151
TGAGCGC252.731109E-670.000012
ATCCTGT150.002190083370.05
ACGCCTA150.002190083370.04
ACGCTCT150.002190083370.05
ACGCCAG353.450623E-970.05
CGATAAT150.002190083370.03
GCAAGAT309.692303E-870.07
TGTCCTG207.722886E-570.04
AAATTAC150.002190083370.07
TGGTAAT207.722886E-570.03
CACGCTC150.002190083370.04
GTCGCCA401.2369128E-1070.04
Produced by FastQC (version 0.11.5)