FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_B_e-1g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_B_e-1g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22114
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAGG252.7498281E-670.09
TCCTCTA150.002196064570.06
TAGCGCT309.785617E-870.03
AAGTAAT252.7498281E-670.03
GCTAGTC150.002196064570.07
GCTAGTA150.002196064570.07
TACAGGA252.7498281E-670.010
AAATTAC150.002196064570.07
GCTAGCA150.002196064570.07
CGCCCCT150.002196064570.03
CCTGTAC150.002196064570.07
CATCCTG150.002196064570.04
TATAATC150.002196064570.04
ATCTAAT150.002196064570.03
TTATAAT252.7498281E-670.03
CCCGCTA150.002196064570.04
TCTAATT150.002196064570.04
GCTAATT150.002196064570.07
GCTAATC252.7498281E-670.04
AGTAATG252.7498281E-670.04
Produced by FastQC (version 0.11.5)