FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_B_e-1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_B_e-1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9962
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC140.14053402931138326Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTAG150.002160468470.045
CGATAAT207.549324E-570.03
TCCTAGA150.002160468470.06
GGGTAAT150.002160468470.03
GTATCCT150.002160468470.03
CCTGACC150.002160468470.07
GAGTTTA150.002160468470.045
ATAATGT150.002160468470.05
ATTACCT150.002160468470.08
GCGCCCC150.002160468470.02
TAATCAA150.002160468470.06
AGCGCCC150.002160468470.01
GTAATCT150.002160468470.05
GTAATCC207.549324E-570.05
CTGTAGA150.002160468470.046
CGCCGCT150.002160468470.03
GGTAATT150.002160468470.04
GGTAATC207.549324E-570.04
GCATAAT150.002160468470.03
TAAATTA409.302312E-961.256
Produced by FastQC (version 0.11.5)