FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_B_e-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_B_e-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135594
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC15370.13534766826876507Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAT5750.063.2984163
CGTAATT6950.062.9465144
TAATTAC25350.062.5412676
GTAATAT15050.062.322495
TGGTAAT18200.062.301853
TAAATTA37700.062.1984986
AGGTAAT16450.062.1218383
CCGCTAT10200.061.7616355
GGTAATA18250.061.3668144
GTAATTA23000.061.323045
CGGTAAT12950.061.075363
GGTAATT14800.060.7740064
CCATAAT6900.060.356663
CGCTAGC6150.060.3222056
GTAATAC10750.060.229565
GCGCTAG9200.060.1057135
CGCTATG7750.060.061536
GTAATCT14300.059.962055
AAATTAC16950.059.8790327
GTAATGT13900.059.6732945
Produced by FastQC (version 0.11.5)