FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_B_e-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_B_e-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548048
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC7430.1355720666802908Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCT1700.065.880643
TAATTAC18550.063.3945776
AGGTAAT6800.063.307173
CGTAATT4400.062.8392684
TAAATTA26500.062.6021426
GTAATTA14450.062.4897165
TAATAGG2300.062.389686
TGCTAAT2250.062.22063
GGTAATT6800.061.7630964
CTAATTA8100.061.3564265
AAATTAC10800.060.924347
TGGTAAT7700.060.9075053
GGTAATC7200.060.7623024
GTAATAC4150.060.7213065
GGCGCTA3300.060.4529724
AAGCGCT6500.060.306123
ACGCTAG1700.059.7043275
CGCTATT4050.059.6280756
CCCGCTA3350.059.5506824
GCGCTAG3900.059.229235
Produced by FastQC (version 0.11.5)