FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_B_e-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_B_e-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324045
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC17990.13587151494095745Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATTA29400.062.618615
TAATTAC37000.062.4319956
TAAATTA50400.062.3606766
GGTAATT17400.062.355894
GCGTAAT7100.061.6192863
CGCTATT12000.061.2495736
CGCTACT7800.061.025216
AAGCGCT15700.060.6365173
TGGTAAT19200.060.5204053
GGGCGCT6200.060.40283
AGGTAAT19100.060.1042863
CGCTAGT11700.059.52956
GTAATCT16900.059.4374545
GCGCTAG10750.058.9298175
CGTAATT10100.058.9104774
CGGTAAT13500.058.8514373
GTAATAT16000.058.8433385
GCATAAT5800.058.534073
CGCTAGC7200.058.3329246
AAATTAC21300.058.166417
Produced by FastQC (version 0.11.5)