FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_pool190111A_B_e-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_pool190111A_B_e-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213619
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC14600.12030134663349865Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAATT16250.064.615354
TAATTAC42550.064.1597756
AGGTAAT13800.062.3912663
GTAATTA37250.062.3892255
CGGTAAT10500.061.3332943
CGTAATT8300.060.301174
TAAATTA68000.060.1690836
CATAATT16100.059.565184
AAATTAC26750.059.5326777
CTAATTA21850.059.26775
CGCTATG4700.058.829756
TAATTAG22100.058.5972486
GGTAATG8700.058.3332984
TGGTAAT15450.058.2200363
TAATTAT36900.057.95396
CAGGTAA8100.057.4667322
GAGTAAT10800.057.361083
TCCAGGA21200.056.957516
CGCTATT7450.056.8456046
GGTAATC14100.056.5957074
Produced by FastQC (version 0.11.5)