FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Ler_B_e-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Ler_B_e-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1626167
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG11403.8198777E-117.676069368
GTGTAGA10951.2551027E-107.671863669
AAGAGTG20100.07.66232170
ACATACG5250.0090347756.66618913
GAGTGTA12753.4615368E-96.588776669
AGAGTGT13802.8358045E-96.34051666
GTAGGGA25250.06.238136370
CGTCGTG60800.06.21761670
GTCGTGG9701.7353597E-56.13414767
CGTGGAG7600.00129205645.987334369
GTGTAGG32750.05.985224770
GGAAAGA27500.05.854666767
TAGGGAA24650.05.82144264
GCGTCGT64200.05.779290769
GGGAAAG24350.05.749428366
CGGAAGC9801.3180007E-45.71475570
CGTGTAG35150.05.676141769
GAAAGAG29300.05.614795768
AAAGAGT28600.05.507445369
TGTAGGG29950.05.49293970