FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Ler_B_e-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Ler_B_e-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069518
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAG500.007208855428.0010366
ACCCTAC1500.005935271713.9998633
AGAGCGG2350.00924599110.42640370
GGCTCGA2350.00925560610.4249429
AGAGTGT10353.6379788E-118.1166269
TCGTGTT6201.2644001E-57.90388670
GAGTGTA10302.2773747E-97.47635270
TAGGGAA22550.07.450732770
GTAGGGA22150.07.269230469
AAGAGTG13553.6379788E-116.974752468
AGTGTAG8602.6637535E-66.919182370
TGTAGGG25900.06.892467568
GGGAAAG17350.06.657616669
CTTCCAT6450.00135122796.51156361
GTGTAGG28450.06.274395567
AGGGAAA24800.05.92791468
GTGTAGA7850.00190095665.796662368
GGAAAGA21753.6379788E-125.632654770
CGTGTAG30750.05.577701670
TGTATGG8450.00455103635.38506570
Produced by FastQC (version 0.11.5)