FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Ler_B_e-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Ler_B_e-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2292804
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG2100.004007651511.66725570
CGCGACG2200.005678100511.13473921
AGAGCGG4401.6065496E-59.54593770
ATCTCGG6653.7896953E-67.895135470
GATCTCG7805.7154284E-77.628590670
AGTGTAG18850.07.613116770
GAGTGTA21350.06.885593469
GGGAAAG36300.06.846075569
TAGGGAA43600.06.502621770
AGAGTGT23150.06.50140868
CGTGTAT15904.0017767E-116.383970370
TCGTGTT14309.385985E-106.36395770
GTAGGGA44250.06.248903369
AAGAGTG29600.06.14895970
GTGTAGA16751.5097612E-106.06000769
GTCGTGG11701.3488334E-65.98320870
GTGTAGG55050.05.785941670
AGGGAAA47000.05.73433268
GAGTCGT8558.426605E-45.731283769
TGTAGGG50850.05.644335768
Produced by FastQC (version 0.11.5)