FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Ler_B_e-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Ler_B_e-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2955804
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG2356.8487466E-411.915565570
GGGAAAA21900.09.11010270
CGCGTGC4000.0060944467.875443540
AGAGCGG5351.5887851E-47.85090970
GCGACGT4955.960011E-47.77821665
ACCGCGT4500.00201220957.778215438
AGTGTAG21750.07.241787470
AGCGACG5650.00242736876.81454364
AGAGTGT30500.06.54124169
CGTCGTT9802.9713938E-66.428933670
GAGTGTA27350.06.39900668
AAGAGTG34550.06.281212368
TCGGAAC6750.00225739236.222572370
GTGTAGA21700.06.12927469
TCGTGTT18305.456968E-126.12056370
GTGTAGG81850.05.94413470
GGAAGAC10701.2380495E-55.888181770
GGGAAAG46500.05.64547967
TAGGGAA65950.05.625790670
AGGGAAA70950.05.62399570
Produced by FastQC (version 0.11.5)