FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_input_e-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_input_e-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448031
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTA1600.00909308913.125977537
AGCTAGC3100.00683678649.0329343
CTTAACG3150.0077979578.8892441
ATATACG5151.0630201E-58.8353041
CTATAGG4350.00144000888.0462981
AGGATAC9805.4709744E-87.14166318
CGGTTGC5050.0062034356.93049160
TTACGCC6107.1791274E-46.885045530
CTAAGAT6954.3884147E-46.54702431
CTTATCT9157.028615E-66.5029921
ATATGAC20650.06.44093561
CTATATG9258.321169E-66.4326891
ATCTTCG6000.0045540416.4169231
CTATCAT8802.7636508E-56.36389061
AGTACAA7257.325743E-46.2761131
ATTCGTT8409.9724464E-56.25024941
CAAGGAT12409.246651E-86.208638716
GGATACA10752.0347507E-66.18501219
TCTGTTC7409.4027095E-46.147620715
CTTAGAT8104.2159393E-46.04962441
Produced by FastQC (version 0.11.5)