FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_input_e-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_input_e-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914312
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCC657.9612085E-426.92126863
GACCTAC750.001831712223.33559657
TCGGTGG2050.00334446311.95172366
GTAATAC3100.0068315039.033133556
CTAAGAG3650.00266857868.6295571
CTTAGTA4150.0084716037.58985141
TATGAGT16000.07.43700032
GGAGTCG6653.213664E-57.367925625
CTATATT10151.3536919E-87.24089341
ATATTGC5100.00682133256.8622841
ATGAGTC13805.638867E-116.84774073
GAGTCTT13856.184564E-116.82301955
AGGCTTA5200.0082123056.731789646
ATACCTT5750.00291734056.6952021
ATTAGAG6000.00455468476.41623541
CATTCTC6100.00541095066.31105141
GAGTCGA8409.969711E-56.2495826
TGGAGTC8451.08177686E-46.212600724
ATATTAG10155.2534906E-66.206481
GCGGTTT10256.150198E-66.14660165
Produced by FastQC (version 0.11.5)