FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_input_e-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_input_e-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1635619
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGG700.00122678624.99996811
TGGTCGC2254.7507905E-412.444427570
GGTGGTC3702.03146E-611.351336568
CGGTGGT3652.1638914E-510.54793266
GTGGTCG3004.4404168E-410.49998669
GAGTCAC2900.0039504319.6551637
TCGGTGG3100.0068418329.03224765
CCGAAGT4050.0068126237.77776762
CGCAACC15350.07.75290746
CAACCGG5552.4268804E-47.568020348
GCAACCC8907.6506694E-87.47235747
ATCCTTA8855.5257624E-77.11863471
TGACTTG23650.07.1035854
ACGGACT5000.0056322256.999990563
GACTTGA22100.06.9683175
ATGACTT27150.06.9613173
ATATGAC27850.06.91201971
CCGCAAC18800.06.888499745
TATGACT27150.06.83240372
ACCGCAA21150.06.78527644
Produced by FastQC (version 0.11.5)