FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_input_e-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_input_e-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990273
Sequences flagged as poor quality0
Sequence length76
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCG700.001226498325.00129953
TAAGGGC1950.002301699212.5638093
CTCGGTG3351.05575615E-410.44804365
GTGGTCG3500.00182347939.00024267
GCAACCC10303.274181E-118.15596947
GGTGGTC3900.0048533878.07734368
CTTAGAG6553.0565843E-68.015081
GCAACCG6401.9383902E-57.65549416
CAACCCG9402.5349436E-87.447196548
CGCAACC17750.07.29633846
CCCGATC10101.2345481E-87.277606551
ACCGCAA26000.07.13516644
TGACTTG24850.07.04208954
ATATGAC32600.06.87100551
ACCCGAT10854.869071E-86.77454650
CCGCAAC22850.06.74012645
GACTTGA23750.06.6314245
TTACGCC7401.3104477E-46.6214675
AAACCGC27100.06.587224542
AACCGCA29300.06.570472743
Produced by FastQC (version 0.11.5)