FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_UB_e3-1i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_UB_e3-1i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184592
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCCG200.006940816552.50386441
CCTTAAG450.004297636431.113455
CTGATCC450.004297636431.113448
ATATTGC850.003783221820.5841711
TGAAAGG900.005266435419.4406071
ATATTCA1300.002284030416.1506581
GCTTGTT1350.002932642615.55670158
GAAGGAG1450.00471762914.48382545
GATTTTC1708.124808E-414.4128245
GAAGCAA1800.0012515813.61211234
ATATAAC1550.00735323213.5457121
CTATATT2250.0066744610.8867391
CCATGAA2250.0066744610.88673919
ATATATG3156.9089804E-49.9980271
Produced by FastQC (version 0.11.5)