FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_UB_e3-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_UB_e3-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611943
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCC500.007205969728.00143668
GTTTCGC950.007208108418.42049454
CCATGCT1250.001761680216.7994914
CTTAACA2350.009246211510.4252161
CTATTAC2350.009246211510.4252161
CATCACA2950.00454329939.4912376
CTCTTCA4350.0014380728.04573251
CTCTCTC6151.13325E-57.9672381
CTATATT6351.7367216E-57.71630141
GGTTGCG5451.9630376E-47.706187719
ATATAAG7401.7075772E-57.09437941
ATTTACA6451.756688E-47.0540491
AATATCA6056.5180124E-46.94193841
AATACAT5650.00242181666.81395241
TCTTCCT5150.00748534646.79591045
ATTAGTA6259.438956E-46.7197961
TTGCGGT6803.3561466E-46.690973821
CTTGATT5250.0090123356.6664651
GTTGCGG7105.674682E-46.408256520
CCAAAGT7652.010035E-46.405034529
Produced by FastQC (version 0.11.5)