FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_UB_e3-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_UB_e3-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587235
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGG1400.003748792515.00133254
CGTCGTG1951.4124396E-414.35902768
TAAGTCC1600.00908891513.1250482
GCGTCGT2200.005663266411.13640467
AATAGGG2350.00924313410.425570533
AACCACT3100.0068258789.0330642
ATATGAC7201.697299E-88.7500321
AGTTATG3850.0043137528.1818491
ATACTTA5201.1379058E-48.0769531
TATGACT6906.3651587E-67.6087232
CTATTTC4650.00276936367.52690941
TCTTATC4700.00307499347.44683553
TGACTTG5150.0074818256.7961414
GCGGTTT5850.00348771346.581220665
AACAAGG6550.00160385766.412236728
TGAGTCT8953.5230332E-56.2570064
AGATATA8501.1689169E-46.1764931
TATACTC8154.5475696E-46.0122923
ATTTATA13608.8526576E-85.91913941
ATTAATG7300.0053926225.75344561
Produced by FastQC (version 0.11.5)