FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_UB_e3-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_UB_e3-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156831
Sequences flagged as poor quality0
Sequence length76
%GC35

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACC305.8637984E-446.66666469
AGTTAGC500.00719169227.99999839
TGCAAAG700.001221834925.00000219
ATATGAT2459.6293015E-411.4285721
TTGTGTC2200.00563207611.13636360
CTTCTTC3950.0053885137.9746841
ATATATT5850.00344817646.58119631
Produced by FastQC (version 0.11.5)