FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_UB_e2-1i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_UB_e2-1i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57170
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACCA200.006918451752.561
GTCTCTT305.832373E-446.66666811
TGGAATG350.00124578440.00000456
TCCTTCA350.00124578440.00000449
TATGAGT850.0037475120.5882362
TACACCA900.00521695719.44444369
GAAGGTT900.00521695719.44444367
ACACCAT950.00712915718.42105170
TTATAAT1000.00958147117.52
ATTATAA1250.001735093316.8000011
Produced by FastQC (version 0.11.5)